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University of California
Berkeley | 1999 | B.A.
Downers Grove, IL
Downers Grove, IL
Chicago College of Optometry
College of Dental Medicine-Illinois
College of Graduate Studies - IL
College of Health Sciences - IL
Biomedical Sciences (M.A.)
Physician Assistant Studies
Human Neuroscience for PA Program
Human Neuroscience for Master of Arts in Biomedical Sciences Program
Human Gross Anatomy for Master of Arts in Biomedical Sciences Program
Excess secretion of the steroid hormone aldosterone from the adrenal cortex is associated with hypertension, stroke, myocardial infarction and other cardiovascular pathologies. At the cellular level, steroid production relies on complex signal transduction pathways that include fluctuations of cytoplasmic calcium. Using live-cell imaging with the calcium-sensitive fluorescent probe Fluo-4 in a human adrenocortical carcinoma cell line (HAC15), we previously demonstrated that activation of M3 muscarinic receptors stimulates intracellular calcium oscillations and enhances aldosterone production (Malaiyandi et al., 2018). More recent work using fluorescence Ca2+ imaging, RT qPCR, and radiolabeled binding assays suggests that autonomic regulation of intracellular Ca2+ depends on an interplay of M3 muscarinic and ⍺2A adrenergic receptors. We are currently investigating potential mechanisms of cooperation between these receptor subtypes.
Students interested in working with us should know that fluorescence methodologies and equipment are highly amenable to novice researchers, for a number of reasons: (i) the necessary devices are easy to operate, therefore researchers can obtain data after brief training periods; (ii) data generation is observed real-time, which allows the luxury and gratification of immediate experimental evaluation; and (iii) a vast array of available fluorescent probes permits the measurement of a large variety of cellular processes including ion fluxes, membrane potential, and mechanisms of cell death. We have mentored dozens of graduate and undergraduate students from all types of health sciences programs. Most have presented their work at regional or national meetings, and many are co-authors on posters and publications. Please contact us if you are interested.
LM Malaiyandi, H Sharthiya, AN Barakat, JR Edwards, KE Dineley (2019) Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line. Biometals 32(6):951-964.
JS Borchert, *B Wang, *M Ramzanali, LM Malaiyandi, AB Stein, KE Dineley (2019) A comparison of adverse events associated with insomnia drugs in patient online reviews and the FAERS database. Journal of Medical Internet Research. 21(11): e13371.
KE Dineley, LM Malaiyandi (2018) Histamine and Antihistamines. In: Brody’s Human Pharmacology, 6th Edition (Edited by L Wecker & RJ Theobald)
KE Dineley, LM Malaiyandi (2018) Respiratory Disorders and Their Treatment (Includes asthma and COPD). In: Brody’s Human Pharmacology, 6th Edition (Edited by L Wecker & RJ Theobald)
LM Malaiyandi, LA Potempa, *N Marschalk, P Jungsuwadee, KE Dineley (2018) Alkylating agent cytotoxicity associated with O6-methylguanine. In: Apoptosis & Beyond: The Many Ways Cells Die (Edited by J Radosevich)
LM Malaiyandi, H Sharthiya, *N Surachaicharn, *Y Shams, *M Arshad, C Schupbach, PG Kopf, KE Dineley. (2018) M3-subtype muscarinic receptor activation stimulates intracellular calcium oscillations and aldosterone synthesis in human adrenocortical HAC15 cells. Mol Cell Endocrinol 478:1-9.
LM Malaiyandi, H Sharthiya, KE Dineley (2016) Fluorescence detection of intracellular cadmium with Leadmium Green. Biometals 29(4): 625-635.
S Kurup, KE Dineley, LM Malaiyandi, R Adewuya, LA Potempa (2013) Drugs to Treat Head and Neck Cancers: Mechanisms of Action. In: Head & Neck Cancer: Current Perspectives, Advances, and Challenges (Edited by J Radosevich)
Member, Society for Neuroscience
Secretary-Elect, Chicago chapter of Society for Neuroscience
Member, American Society for Pharmacology & Experimental Therapeutics
Member, Endocrine Society
Outstanding Faculty Service Award, Francis Marion University
Inducted to Phi Kappa Phi Honor Society
American Heart Association Predoctoral Fellowship