Kolla Kristjansdottir Ph.D.

Associate Professor



KristjansdottirMidwestern University
College of Health Sciences
Biomedical Sciences

203-E Science Hall
555 31st St.
Downers Grove, IL 60515
Office: 630-515-6431
e-mail: kkrist@midwestern.edu

 

 

EDUCATION
  

B.S. Biochemistry University of Iceland 1997
M.S. Biochemistry University of Iceland 1999
Ph.D. Biochemistry Duke University 2005

Research Summary 

The long-term goal of our lab is to identify novel biomarkers and drug targets for treatment of cancer. We identify potential biomarkers and drug targets in cancer cell lines using global mass spectrometry screens. We then examine the role of specific proteins by perturbing protein levels and/or function including knock-downs, overexpression and mutagenesis. The biological role of the proteins is then measured using a wide range of methods including assays for proliferation, migration and localization using fluorescent microscopy.  Our lab is currently focused on two types of cancer, Neuroblastoma, a pediatric cancer, and breast cancer. Our results will help illuminate the role of several proteins in cancer progression and potentially identify new biomarkers or cancer drug targets.

Selected Publications (See PubMed results)

 
Kristjansdottir, K. and Rudolph, J. A fluorescence polarization assay for native protein substrates of kinases. Anal Biochem. 2003. 316,  pp. 41-49.

Sohn, J., Kristjansdottir, K., Safi, A., Parker, B., Kiburz, B. and Rudolph, J. Remote hot spots mediate protein substrate recognition for Cdc25 phosphatase. PNAS. 2004. 101, pp. 16437-41.

Sohn, J., Parks, J., Buhrman, G., Brown, P., Kristjansdottir, K., Safi, A., Edelsbrunner, H., Yang, W., Rudolph, J. Experimental validation of the docking orientation of Cdc25 with its Cdk2/CycA protein substrate. Biochemistry. 2005. 44, pp. 16563-73.

Kristjansdottir, K., Safi, A., Shah, C. and Rudolph, J. Autophosphorylation of Ser66 on Xenopus Myt1 is a Prerequisite for Meiotic Inactivation of Myt1. Cell Cycle. 2006. 5, pp. 421-427.

Wysocki, R., Javaheri, A., Kristjansdottir, K., Sha, F. and Kron, S.J. CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage. Nature Struct and Mol Bio. 2006. 13, pp. 908-14.

Kristjansdottir, K., Wolfgeher, D., Lucius, N., Angulo, D.S., and Kron, S.J. Phosphoprotein profiling by PA-GeLC-MS/MS. J. Proteome Research, 2008. 7, pp. 2812-24.

Volchenboum, S.L., Kristjansdottir, K., Wolfgeher, D., and Kron, S.J. Rapid validation of Mascot search results via stable isotope labeling, pair picking and deconvolution of fragmentation Patterns. Mol Cell Proteomics. 2009. 8, pp. 2011-2022

Truman A.W., K. Kristjansdottir, D. Wolfgeher, N. Hasin, S. Polier, H. Zang, S. Perrett, C. Prodromou, G.W. Jones and S.J. Kron. CDK-dependent Hsp70 phosphorylation controls G1 cyclin abundance and cell cycle progression. Cell. 2012. 151, pp. 1308-1318.

Truman, A.W., Kristjansdottir, K., Wolfgeher, D., Ricco, N., Mayampurath, A., Volchenboum, S.L., Clotet, J., and S. J. Kron. Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase. Journal of Proteomics. 2015. 112, pp. 285-300. PMID: 25452130

Knecht-Sabres LJ, Gunn JF, Conroy C, Getch SE, Cahill SM, Lee MM, Ciancio MJ, Jaskolski J, Palmisano L, and Kristjansdottir K. Effectiveness of an Interprofessional Education Event for Graduate Health Professional Students. The Internet Journal of Allied Health Sciences and Practice. 2016 Oct 21;14(4), Article 2.

Holland KM,  Rosa SJ, Kristjansdottir K, Wolfgeher D, Franz BJ, Zarrella TM, Kumar S, Sunagar R, Singh A, Bakshi CS, Namjoshi P, Barry EM, Sellati TJ, Kron SJ, Gosselin EJ, Reed DS & Karsten R. O. Hazlett. Differential Growth of Francisella tularensis, which alters expression of virulence factors, dominant antigens, and surface-carbohydrate synthases, governs the apparent virulence of Ft SchuS4 to immunized animals. Frontiers in Microbiology. 2017 8:1158:1-17. 

BOOK CHAPTERS: 
Kolbrun Kristjansdottir, Chapter 12: Strategies and Challenges in Measuring Protein Abundance Using Stable Isotope Labeling and Tandem Mass Spectrometry, Tandem Mass Spectrometry - Applications and Principles, Dr Jeevan Prasain (Ed.), Pages: 235-258, 2012. ISBN: 978-953-51-0141-3, InTech.