Medha Joshi, Ph.D.

Assistant Professor, Member Nanomedicine Center of Excellence in Translational Cancer Research


Dr. Joshi

Chicago College of Pharmacy
Department of Pharmaceutical Sciences
Midwestern University
365 Alumani Hall
555 31st St.
Downers Grove, IL 60515

Office: 630-515-6963
e-mail: mjoshi@midwestern.edu

EDUCATION (Curriculum Vitae)

B.Pharm. Pharmacy Pune University (India) 2000
M. Pharm Pharmaceutics Pune University (India) 2002
Ph.D. Pharmaceutics Intitute of Chemical technology, Mumbai University (India) 2006

EXPERIENCE

Research Scientist (December 2010-March 2011)

At Ocean Nanotechnology, Springdale, Arkansas. Formulated nanocolloids, inorganic nanoparticles, conjugated proteins, antibodies, biopolymers (e.g. chitosan) and other targeting agents to iron oxide nanoparticles and quantum dots. Launched lipid nanocolloid range of products for bismuth as imaging agent. Wrote proposals for federal funding.

Post doctoral fellow (November 2008- June 2010)

At Faculty of Science, Department of Pharmaceutics, Utrecht University, Utrecht, The Netherlands, with the research group of Prof. Gert Storm. Project entitled "The glycomics approach for the treatment of cancer". Formulated various types of stimuli sensitive liposomes which are targeted towards the antigen presenting dendritic cells. These liposomes encapsulated with cancer antigens can be used as a cancer vaccine. After successful in vitro and in vivo results the projects aims to scale up this liposome vaccine and for further clinical assesment.

DAAD Post Doctoral fellow (October 2007- September 2008)

Free University of Berlin, Germany with research group of Prof. Rainer H. Müller. Project entitled "Development of Nanocrystals and Nanostructured Lipid Carriers for the delivery of drugs and Cosmeceuticals".  Experience in working for industries (e.g. Amore Pacific, South Korea) interested in making lipid nanoparticles for cosmetics use like sunscreens and other natural products for oral and topical delivery.

Visiting Fellow (March 2007-May 2007)

Tata Institute of Fundamental Research (TIFR), Mumbai, India, with the research group of Prof. Shobhona Sharma. Project entitled 'Genetic polymorphism of host molecules involved in immunity and immunoregulation in Human Filariasis'. Experience in molecular biology techniques like polymerase chain reaction, gel electrophoresis, flow cytometry, ELISA, searching gene database etc.


 

RESEARCH SUMMARY

Targeted drug delivery using nanoparticles

Advances in targeted drug delivery have led to drug formulations that have improved tissue-specific, as well as cell-specific accumulation of therapeutic molecules in the hope of improved efficacy and reduced side effects. My research lab focuses on development of "nanoparticulate" based formulations for the said purpose. Currently I have two projects going on in the lab:

1.     Intracellular delivery of bortezomib using mitochondria targeted ceramide liposomes

In this project we propose development of intracellular delivery of bortezomib through mitochondria targeted ceramide liposomes. Using various osteosarcoma and murine osteoblast precursor cell lines, we will determine the effect of bortezomib and bortezomib liposomes on the metabolic activity of the cells in a time- and dose-dependent manner. The metabolic activity of the cells is also indicative of cellular apoptosis. It is hoped that through use of liposome delivery the bortezomib therapy will be more efficient (thus reducing the number of doses and dosing frequency required) and might also decrease the incidence of the dose limiting toxicity, e.g peripheral neuropathy

2.     Design and development of a clinically acceptable, long circulating liposomal delivery system for IRL-1620 and its evaluation in cerebral ischemia rat models.

In the present study, we propose to test the hypothesis that polyethylene glycol (PEG)ylated liposomal delivery of ETB receptor agonist, IRL-1620 may be efficacious in the treatment of cerebral ischemia. PEGylated liposomes encapsulating IRL-1620 with and without a cationic charge and "targeted" PEGylated liposomes to the transferrin receptor on the brain endothelium will be formulated. Further, these nanocarriers will be tested for their neuroprotection efficiency on pheochromocytoma cells in vitro. The liposomal nanocarriers of IRL-1620 will be elucidated for their ability to accumulate in the brain and amelioration of infarct volume, neurological deficit and motor functions in vivo, in a cerebral ischemia rat model. This study will help in generating the proof of concept that by delivering IRL-1620 in long circulating PEGylated liposomal nanocarriers, higher efficiency in dose and dosing frequency can be achieved. This will have a tremendous clinical significance in the management of stroke, wherein restoration of blood flow and attenuation of secondary damage to the penumbral area by IRL-1620 will be achieved for a longer period of time with fewer side effects and at a higher efficacy.

Patents

1. Vandana Patravale, Medha Joshi and Shobhona Sharma Indian patent entitled "Lipid nanoparticulate dosage forms of antiparasitics and antiinfectives" Patent number 248977, date of filing 28th June 2006, patent granted.

2. Vandana Patravale, Soniya Jain, Medha Joshi, Priyanka Prabhu and Shobhona Sharma Indian patent entitled "Nanodrug Delivery Based On Combination Therapy For Treating Parasite Infections" patent application no. 3567/MUM/2013.

Book Chapter

1. Invited to write a book chapter Patravale V., Joshi M., Nanocarriers for imaging applications, in: F. Monzer (Ed.) Colloids in Drug Delivery, CRC Press, Boca Raton, 2010, pp. 563-611. 

2. Invited to write a book chapter Joshi M, Chuagule M., "Liposomes based drug delivery for the treatment of lung cancer" Encyclopedia of Nanoscience and Nanotechnology, American Scientific publishers to be published in 2016.

Original Research Articles

1. Joshi M and Patravale V (2006) Formulation and evaluation of Nanostructured Lipid Carrier (NLC)-based gel of Valdecoxib. Drug Dev Ind Pharm 32:911-918.

2. Shah K, Date A, Joshi M and Patravale V (2007) Solid lipid nanoparticles (SLN) of tretinoin: potential in topical delivery. Int J Pharm 345:163-171.

3. Joshi M, Pathak S, Sharma S and Patravale V (2008) Design and in vivo pharmacodynamic evaluation of nanostructured lipid carriers for parenteral delivery of artemether: Nanoject. Int J Pharm 364:119-126.

4. Joshi M, Pathak S, Sharma S and Patravale V (2008) Solid microemulsion preconcentrate (NanOsorb) of artemether for effective treatment of malaria. Int J Pharm 362:172-178.

5. Joshi M and Patravale V (2008) Nanostructured lipid carrier (NLC) based gel of celecoxib. Int J Pharm 346:124-132.

6. Shah K, Joshi M and Patravale V (2009) Biocompatible microemulsions for fabrication of glyceryl monostearate solid lipid nanoparticles (SLN) of tretinoin. J Biomed Nanotechnol 5:396-400.

7. Joshi M, Unger W, van Beelen A, Bruijns S, Litjens M, van Bloois L, Kalay H, van Kooyk Y and Storm G (2011) DC-SIGN mediated antigen-targeting using glycan-modified liposomes: formulation considerations. Int J Pharm 416:426-432.

8. Patil S, Joshi M, Pathak S, Sharma S and Patravale V (2012) Intravenous beta-artemether formulation (ARM NLC) as a superior alternative to commercial artesunate formulation. The Journal of antimicrobial chemotherapy 67:2713-2716.

9. Unger W, van Beelen A, Bruijns S, Joshi M, Fehres C, van Bloois L, Verstege M, Ambrosini M, Kalay H, Nazmi K, Bolscher J, Hooijberg E, de Gruijl T, Storm G and van Kooyk Y (2012) Glycan-modified liposomes boost CD4+ and CD8+ T-cell responses by targeting DC-SIGN on dendritic cells. Journal of controlled release 160:88-95.

10. Jain S, Basu H, Prabhu P, Soni U, Joshi M, Mathur, D, Patravale V, Sharma S and Pathak S (2014) Parasite Impairment by Targeting Plasmodium-Infected RBCs Using Glyceryl-dilaurate Nanostructured Lipid Carriers. Biomaterials 35: 6636-6645.

11. Kain V, Ingle K, Prabhu S, Serhan C., Joshi M, and Halade G (2015) Resolvin D1 activates inflammation resolving response at splenic and ventricular site following myocardial infarction leading to improved ventricular function, Journal of Molecular and Cellular Cardiology 84: 24-35, 2015.

12. Venkatesan N, O'Donnell J N, Joshi M, Nguyen H, Rhodes N, Chapman R. and Scheetz M, A simple, rapid, accurate, and precise method of measuring vancomycin concentrations in rat plasma via high-performance liquid chromatography (HPLC) communicated to Biomedical Chromatography B, June 2015.

13. Joshi M, Oesterling B., Wu C., Gwizdz N., Pais G., Briyal S. and Gulati A. (2016) Evaluation of liposomal nanocarriers loaded with ETB receptor agonist, IRL-1620, using cell based assays, Neuroscience, 312, 141-152.

Review Articles

14. Date A, Joshi M and Patravale V (2007) Parasitic diseases: Liposomes and polymeric nanoparticles versus lipid nanoparticles. Adv Drug Deliv Rev 59:505-521.

15. Joshi M and Muller R (2009) Lipid nanoparticles for parenteral delivery of actives. Eur J Pharm Biopharm 71:161-172.

16. Joshi M, Unger W, Storm G, van Kooyk Y and Mastrobattista E (2012) Targeting tumor antigens to dendritic cells using particulate carriers. Journal of controlled release 161:25-37.

17. Prabhu P, Patravale V and Joshi M (2012) Nanocarriers for Effective Topical Delivery of Anti-Infectives. Current Nanoscience 8:491-503.

18. Mehendale R, Joshi M and Patravale VB (2013) Nanomedicines for treatment of viral diseases. Critical reviews in therapeutic drug carrier systems 30:1-49.

19. Oesterling B, Gulati A and Joshi M (2014) Nanocarrier Based Approaches for Treatment and Detection of Alzheimer's disease. Journal of Nanoscience and Nanotechnology 14:137-156.

20. Li W, Joshi M, Singhania S, Ramsey K, Murthy A (2014) Peptide Vaccine: Progress and Challenges, published in the special issue: Peptide Vaccine, Vaccines 2, 515-536.

21. Prabhu R, Patravale V and Joshi M (2015) Polymeric nanoparticles for targeted treatment in oncology: Current insights. International Journal of Nanomedicine, 10, 1001-1018.

Other Websites:

https://www.linkedin.com/in/medhajosh
orcid.org/0000-0002-0280-0349
https://www.researchgate.net/profile/Medha_Joshi3

CURRENT TEACHING ACTIVITIES

Chicago College of Pharmacy (CCP):