Nagaraj Vinay J, Ph.D.

Assistant Professor

Midwestern University
Department of Biochemistry
19555 59th Avenue

Glendale, Arizona 85308 

Office: Agave building - room #201L, phone - (623) 572-3795
Lab: Foothills Science Center - room #141, phone - (623) 537-6086



My research interests are multidisciplinary, and include bioengineering, cellular and molecular biology approaches for


PhD (2004) - University of Basel, Switzerland


Assistant Professor (July 2012 onwards)
Department of Biochemistry, Midwestern University

Research Scientist (July 2010 to June 2012)
Center for Bioelectronics and Biosensors, The Biodesign Institute at Arizona State University 

Senior Biodesign Researcher (Feb 2007 to June 2010)
Center for Applied Nanobioscience, The Biodesign Institute at Arizona State University

Postdoctoral Researcher, (June 2004 to Jan 2007)
Center for Proteins and Peptides for Therapeutics/GT, The Biodesign Institute at Arizona State University 


  1. Valekunja RB, Kamakoti V, Peter A, Phadnis A, Prasad S, and Nagaraj VJ* (2016) The Detection of Papaya Ringspot Virus Coat Protein Using an Electrochemical Immunosensor. Analytical Methods: in press
  2. Romney E, Nagaraj VJ, Kafer A (2016) A clinical case of clozapine-induced fatal diabetic ketoacidosis. Clinical Medicine Insights: Psychiatry 7:1-5
  3. Romney E, Nagaraj VJ (2015) In vitro model systems to investigate drug resistance mechanisms in pancreatic cancer cells. Advances in Biological Chemistry 5: 286-292
  4. Nagaraj VJ, Sun X, Mehta J, Martin M, Ngo T and Dey S (2015) Synthesis, characterization and in vitro drug delivery capabilities of (Zn, Al)-based layered double hydroxide nanoparticles. Journal of Nanotechnology 2015: 350370 ( 
  5. Brownlow B, Nagaraj VJ, Nayel A, Joshi M and Elbayoumi T (2015) Development and in vitro evaluation of vitamin E-enriched nanoemulsion vehicles loaded with Genistein for chemoprevention against UVB-induced skin damage. Journal of Pharmaceutical Sciences 104(10): 3510-3523
  6. Nagaraj VJ, Jacobs M, Vattipalli KM, Annam VP, Prasad S (2014) Nanochannel-Based Electrochemical Sensor for the Detection of Pharmaceutical Contaminants in Water. Environmental Science: Processes and Impacts 16: 135-140
  7. Jacobs M, Nagaraj VJ, Mertz T, Selvam AP, Ngo T and Prasad S (2013) An electrochemical sensor for the detection of antibiotic contaminants in water. Analytical Methods 2013(5): 4325-4329
  8. Wang W, Yang Y, Wang S, Nagaraj VJ, Liu Q, Wu J and Tao NJ (2012) Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells. Nature Chemistry 4: 846-853
  9. Eaton S, Thirstrup D, Lake D, Wiktor P, Nagaraj VJ* (2011) Efficacy of TRAIL treatment against HPV16 infected cervical cancer cells undergoing senescence following siRNA knockdown of E6/E7 genes. Biochemical and Biophysical Research Communications  405(1):1-6
  10. Wang W, Foley K, Shan X, Wang S, Eaton S, Nagaraj VJ, Wiktor P, Patel U, and Tao NJ (2011) A Study of Single Cells and Intracellular Processes: Electrochemical Impedance Microscopy Based on Plasmonics. Nature Chemistry 3: 251-257
  11. Nagaraj VJ*, Eaton S, Wiktor P (2010). NanoProbeArrays for the Analysis of Ultra-low Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology. Journal of the Association for Laboratory Automation Apr;16(2):126-33
  12. Nagaraj VJ, Aithal S, Eaton S, Bothara M, Wiktor P, Prasad S (2010). NanoMonitor: Miniature Electronic Biosensor for Glycan Biomarker Detection. Nanomedicine: 5(3): 369-378(10).
  13. Ritsema T, Brodmann D, Diks SH, Bos CL, Nagaraj V, Pieterse CMJ, Boller T, Wiemken A, Peppelenbosch MP (2009). Are small GTPases signal hubs in sugar-mediated induction of fructan biosynthesis. PLoS One. Aug 12;4(8): e6605.
  14. Kilcoyne M, Shah M, Gerlach JQ, Bhavanandan VP, Nagaraj VJ, Smith AD, Fujiyama K, Sommer U, Costello CE, Olszewski N, Joshi L (2009) O-glycosylation of protein subpopulations in alcohol-extracted rice proteins. Journal of Plant Physiology. Feb 15;166(3):219-32.
  15. Nagaraj VJ*, Eaton S, Thirstrup D, Wiktor P (2008). Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis. Biochemical and Biophysical Research Communications 31;375(4):526-30.
  16. Martínez-Noël G, Nagaraj VJ, Calo G, Wiemken A, Pontis H (2007). Sucrose regulated expression of a Ca2+-dependent protein kinase (TaCDPK1) gene in excised leaves of wheat. Plant Physiology and Biochemistry 45(6-7):410-419.
  17. Nagesha N, Gowda PHR, Madhusudana SN, Joshi L, Nagaraj VJ, Kilcoyne M, Devaiah BN, Madhuvanthi R, Vanikulkarni, Saraswathi S, Dinesh A,N, Gowda TKS, Mehamooda K (2007). Genetic transformation of cantaloupe melon (Cucumis melo L.) with the rabies virus glycoprotein gene (PRGSpRgp) and immunization studies in mice. The Journal of Horticultural Science and Biotechnology 82(3):383-386.
  18. Martínez-Noël G, Togneti J, Nagaraj VJ, Wiemken A, Pontis H (2006). Calcium is essential for fructan synthesis induction mediated by sucrose in wheat. Planta 225(1):183-91.
  19. Van Riet L, Nagaraj VJ, Clerens S, Van den Ende W, Wiemken A, Van Laere A (2006). Purification, cloning and functional characterization of a fructan 6-exohydrolase (6-FEH) from wheat (Triticum aestivum L.). Journal of Experimental Botany 57(1):213-223.
  20. Nagaraj VJ, Galati V, Lüscher M, Boller T, Wiemken A (2005). Cloning and functional characterization of a cDNA encoding barley soluble acid invertase (HvINV1). Plant Science 168: 249-258.
  21. Tran N, Baral C, Nagaraj VJ and Joshi L (2005). Knowledge-Based Framework for Hypothesis Formation in Biochemical Networks. Bioinformatics 2005 Sep 1;21 Suppl 2:ii213-ii219.
  22. Elfstrand M, Feddermann N, Ineichen K, Nagaraj VJ, Wiemken A, Boller T, Salzer P (2005). Ectopic expression of the arbuscule-specific gene Mtchit 3-3 stimulates germination of glomalean fungi. New Phytologist 167 (2), 557-570.
  23. Tran N, Baral C, Nagaraj VJ and Joshi L (2005). Knowledge-Based Integrative Framework for Hypothesis Formation in Biochemical Networks. Lecture Notes in Computer Science 3615: 121-136.
  24. Nagaraj VJ, Altenbach D, Galati V, Lüscher M, Meyer AD, Boller T, Wiemken A (2004). Distinct regulation of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker. New Phytologist 161(3): 735-748.
  25. Nagaraj VJ, Riedl R, Boller T, Wiemken A, Meyer AD (2001) Light and sugar regulation of the barley sucrose:fructan 6- fructosyltransferase promoter. Journal of Plant Physiology 158: 1601-1607.

Sequence Publications in EMBL/GENBANK/DDBJ Databases

  1. AJ306962: Hordeum vulgare sucrose:fructan 6-fructosyltransferase gene promoter
  2. AJ585414: Festuca arundinacea sucrose:sucrose 1-fructosyltransferase gene promoter
  3. AJ605333: Hordeum vulgare mRNA for fructan 1-exohydrolase precursor gene
  4. AM075205: Triticum aestivum mRNA for fructan 6-exohydrolase (6-FEH)
  5. AJ567377: Hordeum vulgare mRNA for sucrose:sucrose 1-fructosyltransferase
  6. AJ605333: Hordeum vulgare mRNA for soluble acid invertase (HvSAI)
  7. AJ621356: Triticum aestivum mRNA for putative calcium-dependent protein kinase
  8. AY238969: Medicago truncatula chitinase (chitIII-3) gene, complete cDNA
  9. AY238970: Medicago truncatula chitinase (chitIII-4) gene, complete cDNA